Atypical cadherins Dachsous and Fat control dynamics of noncentrosomal microtubules in planar cell polarity

Dev Cell. 2010 Sep 14;19(3):389-401. doi: 10.1016/j.devcel.2010.08.004.

Abstract

How global organ asymmetry and individual cell polarity are connected to each other is a central question in studying planar cell polarity (PCP). In the Drosophila wing, which develops PCP along its proximal-distal (P-D) axis, we previously proposed that the core PCP mediator Frizzled redistributes distally in a microtubule (MT)-dependent manner. Here, we performed organ-wide analysis of MT dynamics by introducing quantitative in vivo imaging. We observed MTs aligning along the P-D axis at the onset of redistribution and a small but significant excess of + ends-distal MTs in the proximal region of the wing. This characteristic alignment and asymmetry of MT growth was controlled by atypical cadherins Dachsous (Ds) and Fat (Ft). Furthermore, the action of Ft was mediated in part by PAR-1. All these data support the idea that the active reorientation of MT growth adjusts cell polarity along the organ axis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadherins / physiology*
  • Cell Adhesion Molecules / physiology*
  • Cell Polarity*
  • Drosophila Proteins / physiology*
  • Drosophila melanogaster / embryology*
  • Drosophila melanogaster / genetics
  • Gene Expression Regulation, Developmental / physiology
  • Glycogen Synthase Kinase 3
  • Microtubules / metabolism*
  • Morphogenesis
  • Protein-Serine-Threonine Kinases / physiology
  • Signal Transduction
  • Subcellular Fractions
  • Wings, Animal / embryology
  • Wings, Animal / metabolism

Substances

  • Cadherins
  • Cell Adhesion Molecules
  • Drosophila Proteins
  • ds protein, Drosophila
  • ft protein, Drosophila
  • Protein-Serine-Threonine Kinases
  • Glycogen Synthase Kinase 3
  • Par-1 protein, Drosophila