Growth suppression of human laryngeal squamous cell carcinoma by adenoviral-mediated interleukin-12

J Int Med Res. 2010 May-Jun;38(3):994-1004. doi: 10.1177/147323001003800326.

Abstract

This study explored the inhibitory role of the adenoviral-mediated-interleukin (IL)-12 (Ad.mIL-12) gene in the growth of laryngeal squamous cell carcinoma (LSCC). Human epithelial type 2 (Hep-2) cells were transfected with Ad.mIL-12, and IL-12 gene expression of the cells was evaluated. The proliferation and apoptosis of Hep-2 cells in vitro were detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. Experimental tumours in mice were injected intratumourally with the same recombinant adenoviruses and inhibition of tumour growth observed. Apoptosis in Hep-2 xenotransplants was detected using TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labelling) assay and transmission electron microscopy. The expression of IL-12 in Ad.mIL-12 transfected Hep-2 cells was significantly increased. In vitro, Ad.mIL-12 decreased the viability of and increased apoptosis in Hep-2 cells. Increased apoptosis was also seen in vivo. The mean weight and volume of tumours in Ad.mIL-12 treated mice were significantly lower than in the control group. It is concluded that Ad.mIL-12 can suppress LSCC growth and induce apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Apoptosis
  • Carcinoma, Squamous Cell / metabolism
  • Carcinoma, Squamous Cell / pathology*
  • Carcinoma, Squamous Cell / therapy
  • Cell Survival
  • Genetic Therapy / methods*
  • Genetic Vectors*
  • Hep G2 Cells / ultrastructure
  • Hep G2 Cells / virology
  • Humans
  • Interleukin-12 / genetics
  • Interleukin-12 / metabolism
  • Laryngeal Neoplasms / metabolism
  • Laryngeal Neoplasms / pathology*
  • Laryngeal Neoplasms / therapy
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Electron, Transmission
  • Tetrazolium Salts / metabolism
  • Thiazoles / metabolism
  • Transfection
  • Xenograft Model Antitumor Assays

Substances

  • Tetrazolium Salts
  • Thiazoles
  • Interleukin-12
  • thiazolyl blue