Highly sensitive protein detection based on aptamer probe and isothermal nicking enzyme assisted fluorescence signal amplification

Liyun Xue; Xiaoming Zhou; Da Xing

doi:10.1039/c0cc02038a

Highly sensitive protein detection based on aptamer probe and isothermal nicking enzyme assisted fluorescence signal amplification

Chem Commun (Camb). 2010 Oct 21;46(39):7373-5. doi: 10.1039/c0cc02038a. Epub 2010 Sep 6.

Authors

Liyun Xue¹, Xiaoming Zhou, Da Xing

Affiliation

¹ MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631 Guangzhou, China.

PMID: 20820523
DOI: 10.1039/c0cc02038a

Abstract

An isothermal and sensitive fluorescence assay for protein detection using aptamer-protein-aptamer conjugates based on nicking enzyme amplification has been developed, which was combined with magnetic microparticles separation, making this assay suitable for protein detection in biological samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide*
Base Sequence
Fluorescent Dyes
Limit of Detection
Proteins / analysis*
Spectrometry, Fluorescence

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Proteins