Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells

Vet J. 2011 Sep;189(3):361-3. doi: 10.1016/j.tvjl.2010.07.016. Epub 2010 Sep 6.

Abstract

Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7-14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / genetics
  • Acid Phosphatase / metabolism
  • Animals
  • Bone and Bones / metabolism
  • Cells, Cultured
  • Gene Expression Regulation
  • Giant Cells / pathology*
  • Goats
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Leukocytes, Mononuclear / metabolism*
  • Macrophage Colony-Stimulating Factor / genetics
  • Macrophage Colony-Stimulating Factor / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Monocytes / metabolism*
  • Osteoclasts / cytology*
  • Osteoclasts / metabolism
  • Osteoclasts / pathology
  • Peptide Hydrolases / genetics
  • Peptide Hydrolases / metabolism
  • Phenotype
  • RANK Ligand / genetics
  • RANK Ligand / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Tartrate-Resistant Acid Phosphatase

Substances

  • Isoenzymes
  • Membrane Proteins
  • RANK Ligand
  • Macrophage Colony-Stimulating Factor
  • Acid Phosphatase
  • Tartrate-Resistant Acid Phosphatase
  • Peptide Hydrolases