Fragmentation of the Golgi Apparatus Provides Replication Membranes for Human Rhinovirus 1A

Virology. 2010 Nov 25;407(2):185-95. doi: 10.1016/j.virol.2010.08.012. Epub 2010 Sep 9.

Abstract

All viruses with a positive-stranded RNA genome replicate their genomic RNA in association with membranes from the host cell. Here we demonstrate a novel organelle source of replication membranes for human rhinovirus 1A (HRV-1A). HRV-1A infection induces fragmentation of the Golgi apparatus, and Golgi membranes are rearranged into vesicles of approximately 250-500 nm diameter. The newly distributed Golgi membranes co-localize with viral RNA replication templates, strongly suggesting that the observed vesicles are the sites of viral RNA replication. Expression of the HRV-1A 3A protein induces alterations in the Golgi staining pattern similar to those seen during viral infection, and expressed 3A localizes to the Golgi-derived membranes. Taken together, these data show that in HRV-1A infection, the 3A protein plays a role in fragmenting the Golgi complex and generating vesicles that are used as the site of viral RNA replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Golgi Apparatus / metabolism*
  • Golgi Apparatus / ultrastructure
  • Golgi Apparatus / virology
  • HeLa Cells
  • Humans
  • Intracellular Membranes / virology*
  • RNA, Viral / metabolism
  • Rhinovirus / physiology*
  • Viral Proteins / metabolism*
  • Virus Replication*

Substances

  • RNA, Viral
  • Viral Proteins