Inulin prolongs survival of intragastrically administered Lactobacillus plantarum No. 14 in the gut of mice fed a high-fat diet

J Nutr. 2010 Nov;140(11):1963-9. doi: 10.3945/jn.110.128082. Epub 2010 Sep 8.

Abstract

We tested whether a high-fat diet (HFD) impairs the survival of probiotics in mice. In Expt. 1, after feeding either a HFD (62.7% energy) or a normal-fat diet (NFD; 11.1% energy) for 2 d, C57BL/6 mice were i.g. administered Lactobacillus plantarum No. 14. Fecal recovery of viable L. plantarum was significantly decreased 99% by the HFD compared with the NFD. Total bile acid concentrations in the small intestine and cecum were significantly higher (1.5- and 2.2-fold of NFD, respectively) in mice fed HFD than in those fed NFD. Cholic acid and deoxycholic acid significantly reduced the viability of L. plantarum No. 14 in culture experiments. In Expt. 2, after feeding HFD for 2 d, simultaneous administration of inulin (10 mg) with L. plantarum No. 14 significantly increased (100-fold of that without inulin) the fecal recovery of viable L. plantarum. Inulin administration did not alter intestinal bile acid concentrations. In Expt. 3, after feeding HFD for 2 d, mice were i.g. administered either inulin (10 mg) or vehicle and, after 6 h, cecal contents were subjected to culture experiments. Growth of L. plantarum No. 14 was significantly higher in the cecal contents of inulin-administered mice than vehicle-administered mice. Inulin supplementation to cecal contents of vehicle-administered mice significantly enhanced the growth of L. plantarum No. 14. We propose that HFD impairs the survival of probiotics in the gut due to increased bile acid stress and that simultaneous administration of inulin prolongs the survival of probiotics in mice fed HFD.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / analysis
  • Anti-Bacterial Agents / pharmacology
  • Bile Acids and Salts / analysis
  • Cecum / microbiology
  • Cholic Acid / analysis
  • Cholic Acid / pharmacology
  • Colony Count, Microbial
  • Deoxycholic Acid / analysis
  • Deoxycholic Acid / pharmacology
  • Dietary Fats / adverse effects*
  • Feces / microbiology
  • Female
  • Gastrointestinal Contents / chemistry
  • Gastrointestinal Contents / microbiology
  • Intestine, Small / microbiology
  • Inulin / administration & dosage*
  • Inulin / metabolism
  • Lactobacillus / genetics
  • Lactobacillus / growth & development
  • Lactobacillus / isolation & purification
  • Lactobacillus plantarum / drug effects
  • Lactobacillus plantarum / growth & development*
  • Lactobacillus plantarum / isolation & purification
  • Mice
  • Mice, Inbred C57BL
  • Microbial Viability*
  • Osmolar Concentration
  • Probiotics / administration & dosage*
  • Probiotics / metabolism
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Bile Acids and Salts
  • Dietary Fats
  • Deoxycholic Acid
  • Inulin
  • Cholic Acid