A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Chem Commun (Camb). 2011 Jan 7;47(1):397-9. doi: 10.1039/c0cc02229b. Epub 2010 Sep 9.


Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Biomarkers, Tumor / metabolism
  • Biosensing Techniques / methods*
  • Cell Death
  • Cell Line, Tumor
  • DNA Repair
  • HeLa Cells
  • Humans
  • Luciferases / chemistry
  • Luciferases / metabolism*
  • Molecular Conformation
  • Poly Adenosine Diphosphate Ribose / analysis*
  • Poly Adenosine Diphosphate Ribose / metabolism


  • Biomarkers, Tumor
  • Poly Adenosine Diphosphate Ribose
  • Luciferases