Hydrogen sulphide, a novel gasotransmitter involved in guard cell signalling

New Phytol. 2010 Dec;188(4):977-84. doi: 10.1111/j.1469-8137.2010.03465.x. Epub 2010 Sep 9.

Abstract

Hydrogen sulphide (H(2) S) has been proposed as the third gasotransmitter. In animal cells, H(2) S has been implicated in several physiological processes. H(2) S is endogenously synthesized in both animals and plants by enzymes with l-Cys desulphydrase activity in the conversion of l-Cys to H(2) S, pyruvate and ammonia. The participation of H(2) S in both stomatal movement regulation and abscisic acid (ABA)-dependent induction of stomatal closure was studied in epidermal strips of three plant species (Vicia faba, Arabidopsis thaliana and Impatiens walleriana). The effect of H(2) S on stomatal movement was contrasted with leaf relative water content (RWC) measurements of whole plants subjected to water stress. In this work we report that exogenous H(2) S induces stomatal closure and this effect is impaired by the ATP-binding cassette (ABC) transporter inhibitor glibenclamide; scavenging H(2) S or inhibition of the enzyme responsible for endogenous H(2) S synthesis partially blocks ABA-dependent stomatal closure; and H(2) S treatment increases RWC and protects plants against drought stress. Our results indicate that H(2) S induces stomatal closure and participates in ABA-dependent signalling, possibly through the regulation of ABC transporters in guard cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / antagonists & inhibitors
  • ATP-Binding Cassette Transporters / metabolism
  • Abscisic Acid / pharmacology
  • Droughts
  • Glyburide / pharmacology
  • Hydrogen Sulfide / metabolism*
  • Hydrogen Sulfide / pharmacology
  • Impatiens / cytology
  • Impatiens / drug effects
  • Impatiens / metabolism
  • Plant Stomata / cytology*
  • Plant Stomata / drug effects
  • Plant Stomata / metabolism*
  • Potassium Channels, Inwardly Rectifying / antagonists & inhibitors
  • Potassium Channels, Inwardly Rectifying / metabolism
  • Receptors, Drug / antagonists & inhibitors
  • Receptors, Drug / metabolism
  • Signal Transduction* / drug effects
  • Stress, Physiological / drug effects
  • Sulfonylurea Receptors
  • Vicia faba / cytology
  • Vicia faba / drug effects
  • Vicia faba / metabolism

Substances

  • ATP-Binding Cassette Transporters
  • Potassium Channels, Inwardly Rectifying
  • Receptors, Drug
  • Sulfonylurea Receptors
  • Abscisic Acid
  • Glyburide
  • Hydrogen Sulfide