Human odontoblasts express transient receptor protein and acid-sensing ion channel mechanosensor proteins

Microsc Res Tech. 2011 May;74(5):457-63. doi: 10.1002/jemt.20931. Epub 2010 Sep 10.

Abstract

Diverse proteins of the denegerin/epithelial sodium channel (DEG/ENa(+) C) superfamily, in particular those belonging to the acid-sensing ion channel (ASIC) family, as well as some members of the transient receptor protein (TRP) channel, function as mechanosensors or may be required for mechanosensation in a diverse range of species and cell types. Therefore, we investigated the putative mechanosensitive function of human odontoblasts using immunohistochemistry to detect ENa(+) C subunits (α, β, and γ) and ASIC (1, 2, 3, and 4) proteins, as well as TRPV4, in these cells. Positive and specific immunoreactivity in the odontoblast soma and/or processes was detected for all proteins studied except α-ENa(+) C. The intensity of immunostaining was high for β-ENa(+) C and ASIC2, whereas it was low for ASIC1, ASIC3, γ-ENa(+) C, and TRPV4, being absent for α-ENa(+) C and ASIC4. These results suggest that human odontoblasts in situ express proteins related to mechanosensitive channels that probably participate in the mechanisms involved in teeth sensory transmission.

MeSH terms

  • Acid Sensing Ion Channels
  • Adult
  • Female
  • Fluorescent Antibody Technique
  • Gene Expression*
  • Humans
  • Immunohistochemistry
  • Male
  • Membrane Proteins / biosynthesis*
  • Microscopy
  • Middle Aged
  • Nerve Tissue Proteins / biosynthesis*
  • Odontoblasts / metabolism*
  • Sodium Channels / biosynthesis*
  • TRPV Cation Channels / biosynthesis*

Substances

  • ASIC1 protein, human
  • ASIC4 protein, human
  • Acid Sensing Ion Channels
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Sodium Channels
  • TRPV Cation Channels
  • TRPV4 protein, human