Cardiac contractile dysfunction and apoptosis in streptozotocin-induced diabetic rats are ameliorated by garlic oil supplementation

J Agric Food Chem. 2010 Oct 13;58(19):10347-55. doi: 10.1021/jf101606s.

Abstract

Previous studies have suggested that garlic oil could protect the cardiovascular system. However, the mechanism by which garlic oil protects diabetes-induced cardiomyopathy is unclear. In this study, streptozotocin (STZ)-induced diabetic rats received garlic oil (0, 10, 50, or 100 mg/kg of body weight) by gastric gavage every 2 days for 16 days. Normal rats without diabetes were used as control. Cardiac contractile dysfunction examined by echocardiography and apoptosis evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay were observed in diabetic rat hearts. Additionally, a shift in cardiac myosin heavy chain (MHC) gene expression from α- to β-MHC isoform, decreased levels of superoxide dismutase-1 (SOD-1) and cardiac α-actin, and elevated cardiac thiobarbituric acid reactive substances (TBARS) and caspase- and p38-NFκB-leading apoptosis signaling activities were demonstrated in diabetic hearts. However, these diabetes-related cardiac dysfunctions were almost dose-dependently ameliorated by garlic oil administration. In conclusion, garlic oil possesses significant potential for protecting hearts from diabetes-induced cardiomyopathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allyl Compounds / administration & dosage*
  • Animals
  • Antioxidants / analysis
  • Apoptosis*
  • Caspases / metabolism
  • Diabetes Mellitus, Experimental / complications*
  • Diabetes Mellitus, Experimental / drug therapy
  • Diabetic Cardiomyopathies / drug therapy*
  • Diabetic Cardiomyopathies / pathology
  • Diabetic Cardiomyopathies / physiopathology
  • Dose-Response Relationship, Drug
  • Gene Expression
  • In Situ Nick-End Labeling
  • Male
  • Myocardial Contraction*
  • Myocardium / chemistry
  • Myocardium / pathology
  • Myosin Heavy Chains / genetics
  • Phytotherapy
  • Rats
  • Rats, Wistar
  • Sulfides / administration & dosage*

Substances

  • Allyl Compounds
  • Antioxidants
  • Sulfides
  • allyl sulfide
  • Caspases
  • Myosin Heavy Chains