Novel SNPs in the ATP1B2 gene and their associations with milk yield, milk composition and heat-resistance traits in Chinese Holstein cows

Mol Biol Rep. 2011 Mar;38(3):1749-55. doi: 10.1007/s11033-010-0289-6. Epub 2010 Sep 15.

Abstract

Genetic association analysis was applied to examine the effect of the Na(+)/K(+)-ATPase beta 2 subunit (ATP1B2) gene on rectal temperature, milk traits, K(+) levels and Na(+)/K(+)-ATPase (NKA) activity in the red blood cells of 1001 Chinese Holstein cows under normal and heat-stress conditions. We detected two novel single nucleotide polymorphisms, G2258A and C2833T, in the second and fourth introns, respectively, of ATP1B2. G2258A significantly affected milk fat content (P < 0.05) and 305-day milk yield (P < 0.01), but not milk protein content. C2833T significantly affected milk protein content (P < 0.01) and 305-day milk yield (P < 0.05), but not milk fat content. Calculated gene substitution effects suggested that A to G substitution in G2258A, and T to C substitution in C2833T, positively affected milk fat content, 305-day milk yield and somatic cell score, but negatively affected milk protein content. We also detected significant variation in milk fat content, milk protein content, 305-day milk yield and somatic cell scores (P < 0.05 or P < 0.01) among the nine ATP1B2 haplotypes. Under heat-stress, the C2833T polymorphism was significantly related to rectal temperature (P < 0.01), red blood cell K(+) levels, NKA activity and milk yield (P < 0.05). Cows with the TT genotype showed the desirable characteristics of low rectal temperature and red blood cell K(+), low decline rate in milk yield and red blood cell NKA activity. This study suggests that the ATP1B2 single nucleotide polymorphism C2833T is a genetic marker of heat-resistance traits in Chinese Holstein cows.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Alleles
  • Animals
  • Base Sequence
  • Body Temperature
  • Cattle / genetics*
  • China
  • Dairying
  • Erythrocytes / enzymology
  • Female
  • Gene Frequency / genetics
  • Genetic Association Studies*
  • Genotype
  • Hot Temperature*
  • Milk / chemistry*
  • Molecular Sequence Data
  • Polymorphism, Single Nucleotide / genetics*
  • Quantitative Trait, Heritable*
  • Sequence Analysis, DNA
  • Sodium-Potassium-Exchanging ATPase / metabolism

Substances

  • Adenosine Triphosphatases
  • Sodium-Potassium-Exchanging ATPase