Hydrophilic interaction liquid chromatography-tandem mass spectrometry for drug development

Curr Drug Discov Technol. 2010 Sep;7(3):223-31.


Hydrophilic interaction chromatography (HILIC) offers a difference in selectivity as compared to traditional normal phase and reversed-phase liquid chromatography and therefore has great potential for the separation of a variety of pharmaceuticals. This review is devoted to summarizing HILIC coupled to tandem mass spectrometric (MS/MS) methods for the rapid, sensitive and accurate determinations of small molecules employed for supporting drug discovery. The perspectives and challenges on performing HILIC-MS/MS assays are also presented.

Publication types

  • Review

MeSH terms

  • Chromatography, High Pressure Liquid / instrumentation
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Liquid / instrumentation
  • Chromatography, Liquid / methods*
  • Drug Discovery*
  • Hydrophobic and Hydrophilic Interactions
  • Pharmaceutical Preparations / analysis*
  • Phase Transition
  • Tandem Mass Spectrometry / instrumentation
  • Tandem Mass Spectrometry / methods*


  • Pharmaceutical Preparations