PAMP (pathogen-associated molecular pattern)-induced changes in plasma membrane compartmentalization reveal novel components of plant immunity

J Biol Chem. 2010 Dec 10;285(50):39140-9. doi: 10.1074/jbc.M110.160531. Epub 2010 Sep 15.


Plasma membrane compartmentalization spatiotemporally regulates cell-autonomous immune signaling in animal cells. To elucidate immediate early protein dynamics at the plant plasma membrane in response to the bacterial pathogen-associated molecular pattern (PAMP) flagellin (flg22) we employed quantitative mass spectrometric analysis on detergent-resistant membranes (DRMs) of Arabidopsis thaliana suspension cells. This approach revealed rapid and profound changes in DRM protein composition following PAMP treatment, prominently affecting proton ATPases and receptor-like kinases, including the flagellin receptor FLS2. We employed reverse genetics to address a potential contribution of a subset of these proteins in flg22-triggered cellular responses. Mutants of three candidates (DET3, AHA1, FER) exhibited a conspicuous defect in the PAMP-triggered accumulation of reactive oxygen species. In addition, these mutants showed altered mitogen-activated protein kinase (MAPK) activation, a defect in PAMP-triggered stomatal closure as well as altered bacterial infection phenotypes, which revealed three novel players in elicitor-dependent oxidative burst control and innate immunity. Our data provide evidence for dynamic elicitor-induced changes in the membrane compartmentalization of PAMP signaling components.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / immunology*
  • Arabidopsis / microbiology*
  • Arabidopsis Proteins
  • Cell Membrane / metabolism*
  • Detergents / pharmacology
  • Flagellin / chemistry
  • Immune System
  • Immunity, Innate
  • MAP Kinase Signaling System
  • Mass Spectrometry / methods
  • Membrane Microdomains / chemistry
  • Phosphotransferases
  • Plant Leaves / microbiology
  • Proteomics / methods
  • Reactive Oxygen Species
  • Respiratory Burst
  • Vacuolar Proton-Translocating ATPases


  • Arabidopsis Proteins
  • Detergents
  • Reactive Oxygen Species
  • Flagellin
  • FERONIA receptor like kinase, Arabidopsis
  • Phosphotransferases
  • Det3 protein, Arabidopsis
  • Vacuolar Proton-Translocating ATPases