Influence of Moringa oleifera on pharmacokinetic disposition of rifampicin using HPLC-PDA method: a pre-clinical study

Biomed Chromatogr. 2011 Jun;25(6):641-5. doi: 10.1002/bmc.1494. Epub 2010 Sep 15.


The influence of active fraction isolated from pods of an indigenous plant, Moringa oleifera (MoAF) was studied on the pharmacokinetic profile of the orally administered frontline anti-tuberculosis drug rifampicin (20 mg/kg b.w.) in Swiss albino mice. The antibiotic rifampicin alone and in combination with MoAF (0.1 mg/kg b.w.) was administered orally and heparanized blood samples were collected from the orbital plexus of mice for plasma separation at 0, 1, 2, 3, 4 and 5 h, post treatment. Plasma rifampicin concentration, pharmacokinetic parameters and drug metabolizing enzyme (cytochrome P-450) activity were determined. The pharmacokinetic data revealed that MoAF-treated animals had significantly increased rifampicin plasma concentration, C(max), K(el), t(½(a)), t(½(el)), K(a) and AUC as well as inhibited rifampicin-induced cytochrome P-450 activity. In conclusion, the result of this study suggested that the bioavailability-enhancing property of MoAF may help to lower the dosage level and shorten the treatment course of rifampicin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Area Under Curve
  • Biological Availability
  • Chromatography, High Pressure Liquid / methods*
  • Cytochrome P-450 Enzyme System / metabolism
  • Drug Evaluation, Preclinical
  • Drug Synergism
  • Female
  • Male
  • Mice
  • Microsomes, Liver / chemistry
  • Microsomes, Liver / enzymology
  • Moringa oleifera / chemistry*
  • Plant Extracts / pharmacology*
  • Rifampin / blood
  • Rifampin / pharmacokinetics*


  • Plant Extracts
  • Cytochrome P-450 Enzyme System
  • Rifampin