Mucin gene expression and mouse middle ear epithelium

Int J Pediatr Otorhinolaryngol. 2010 Aug;74(8):864-8. doi: 10.1016/j.ijporl.2010.04.014.


Objectives: To investigate the expression of recently identified human mucin genes in an in vitro model of cultured mouse middle ear epithelial cells (MMEEC).

Methods: MMEEC were established, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc6, Muc7, Muc8, Muc9, Muc10, Muc11/12, Muc13, Muc15, Muc16, Muc17, Muc18, Muc19 and Muc20 expression.

Results: Mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc9, Muc10, Muc13, Muc15, Muc16, Muc18, Muc19 and Muc20 were identified and expressed in MMEEC. The genes Muc6, Muc7, Muc8, Muc11/12 and Muc17 were not identified.

Conclusion: Many of the mucin genes that have been recently identified in human MEE and chinchilla MEE are also expressed in MMEEC. There are differences in expression, however, which may have implications in utilizing various animal models for study of middle ear physiology and pathogenesis; specifically as it relates to mucin gene expression.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Ear, Middle / cytology
  • Epithelial Cells / metabolism*
  • Epithelial Cells / physiology
  • Gene Expression Regulation*
  • Humans
  • In Vitro Techniques
  • Mice
  • Models, Animal
  • Mucins / genetics*
  • Mucins / metabolism
  • Otitis Media / genetics
  • Otitis Media / physiopathology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity


  • Mucins