Collagen XV is necessary for modeling of the extracellular matrix and its deficiency predisposes to cardiomyopathy

Circ Res. 2010 Nov 12;107(10):1241-52. doi: 10.1161/CIRCRESAHA.110.222133. Epub 2010 Sep 16.


Rationale: The extracellular matrix (ECM) is a major determinant of the structural integrity and functional properties of the myocardium in common pathological conditions, and changes in vasculature contribute to cardiac dysfunction. Collagen (Col) XV is preferentially expressed in the ECM of cardiac muscle and microvessels.

Objective: We aimed to characterize the ECM, cardiovascular function and responses to elevated cardiovascular load in mice lacking Col XV (Col15a1(-/-)) to define its functional role in the vasculature and in age- and hypertension-associated myocardial remodeling.

Methods and results: Cardiac structure and vasculature were analyzed by light and electron microscopy. Cardiac function, intraarterial blood pressure, microhemodynamics, and gene expression profiles were studied using echocardiography, telemetry, intravital microscopy, and PCR, respectively. Experimental hypertension was induced with angiotensin II or with a nitric oxide synthesis inhibitor. Under basal conditions, lack of Col XV resulted in increased permeability and impaired microvascular hemodynamics, distinct early-onset and age-dependent defects in heart structure and function, a poorly organized fibrillar collagen matrix with marked interstitial deposition of nonfibrillar protein aggregates, increased tissue stiffness, and irregularly organized cardiomyocytes. In response to experimental hypertension, Col15a1 gene expression was increased in the left ventricle of wild-type mice, and mRNA expression of natriuretic peptides (ANP and BNP) and ECM modeling were abnormal in Col15a1(-/-) mice.

Conclusions: Col XV is necessary for ECM organization in the heart, and for the structure and functions of microvessels. Col XV deficiency leads to a complex cardiac phenotype and predisposes the subject to pathological responses under cardiac stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Aging
  • Angiotensin II
  • Animals
  • Atrial Natriuretic Factor / genetics
  • Cardiomyopathies / etiology*
  • Cardiomyopathies / genetics
  • Cardiomyopathies / metabolism
  • Cardiomyopathies / physiopathology
  • Collagen / deficiency
  • Collagen / genetics
  • Collagen / metabolism*
  • Coronary Circulation
  • Disease Models, Animal
  • Echocardiography
  • Elasticity
  • Enzyme Inhibitors
  • Extracellular Matrix / metabolism*
  • Female
  • Gene Expression Profiling / methods
  • Gene Expression Regulation
  • Genotype
  • Heart Ventricles / metabolism
  • Heart Ventricles / physiopathology
  • Hemodynamics
  • Hypertension / chemically induced
  • Hypertension / complications*
  • Hypertension / genetics
  • Hypertension / metabolism
  • Hypertension / physiopathology
  • Male
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microcirculation
  • Microscopy, Electron
  • Microscopy, Video
  • Myocardium / metabolism*
  • Myocardium / ultrastructure
  • NG-Nitroarginine Methyl Ester
  • Natriuretic Peptide, Brain / genetics
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Nitric Oxide Synthase / metabolism
  • Phenotype
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism
  • Telemetry
  • Ventricular Remodeling*


  • Enzyme Inhibitors
  • RNA, Messenger
  • collagen XV, mouse
  • Angiotensin II
  • Natriuretic Peptide, Brain
  • Atrial Natriuretic Factor
  • Collagen
  • Nitric Oxide Synthase
  • NG-Nitroarginine Methyl Ester