Abstract
Human carcinosarcomas of esophagus are uncommon malignant neoplasms that are composed both carcinomatous and sarcomatous components. We established a novel cell line, HN-Eso-1, from the metastatic esophageal spindle cell carcinoma (so-called carcinosarcoma). In this study, we estimated the vascular endothelial growth factors (VEGFs) and VEGF receptors (VEGFRs). Reverse transcription polymerase chain reaction (RT-PCR) studies revealed that VEGF-A, -C, -D and VEGFR-1, -2 were upregulated. Cisplatin reduced the cell viability of HN-Eso-1 cells and VEGF attenuated its effect. These results suggest that expression of VEGF-A, VEGF-C, VEGF-D, VEGFR-1, and VEGFR-2 are involved in the cell's autocrine system and that VEGF protected these cells from the anti-tumor agent.
MeSH terms
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Antineoplastic Agents / pharmacology
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Base Sequence
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Carcinoma / drug therapy
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Carcinoma / genetics
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Carcinoma / metabolism*
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Carcinoma / pathology
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Carcinosarcoma / drug therapy
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Carcinosarcoma / genetics
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Carcinosarcoma / metabolism
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Carcinosarcoma / pathology
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Cell Culture Techniques
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Cell Line, Tumor
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Cisplatin / pharmacology
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DNA Primers / genetics
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Esophageal Neoplasms / drug therapy
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Esophageal Neoplasms / genetics
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Esophageal Neoplasms / metabolism*
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Esophageal Neoplasms / pathology
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Humans
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism
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Receptors, Vascular Endothelial Growth Factor / genetics
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Receptors, Vascular Endothelial Growth Factor / metabolism*
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Vascular Endothelial Growth Factor A / genetics
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Vascular Endothelial Growth Factor A / metabolism*
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Vascular Endothelial Growth Factor C / genetics
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Vascular Endothelial Growth Factor C / metabolism
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Vascular Endothelial Growth Factor D / genetics
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Vascular Endothelial Growth Factor D / metabolism
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Vascular Endothelial Growth Factor Receptor-1 / genetics
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Vascular Endothelial Growth Factor Receptor-1 / metabolism
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Vascular Endothelial Growth Factor Receptor-2 / genetics
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Vascular Endothelial Growth Factor Receptor-2 / metabolism
Substances
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Antineoplastic Agents
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DNA Primers
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RNA, Messenger
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RNA, Neoplasm
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VEGFA protein, human
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VEGFC protein, human
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factor C
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Vascular Endothelial Growth Factor D
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Receptors, Vascular Endothelial Growth Factor
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Vascular Endothelial Growth Factor Receptor-1
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Vascular Endothelial Growth Factor Receptor-2
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Cisplatin