First report of an anti-tumor, anti-fungal, anti-yeast and anti-bacterial hemolysin from Albizia lebbeck seeds

Phytomedicine. 2011 May 15;18(7):601-8. doi: 10.1016/j.phymed.2010.08.009. Epub 2010 Sep 18.


A monomeric 5.5-kDa protein with hemolytic activity toward rabbit erythrocytes was isolated from seeds of Albizia lebbeck by using a protocol that involved ion-exchange chromatography on Q-Sepharose and SP-Sepharose, hydrophobic interaction chromatography on Phenyl-Sepharose, and gel filtration on Superdex 75. It was unadsorbed on both Q-Sepharose and SP-Sepharose, but adsorbed on Phenyl-Sepharose. Its hemolytic activity was fully preserved in the pH range 0-14 and in the temperature range 0-100 °C, and unaffected in the presence of a variety of metal ions and carbohydrates. The hemolysin reduced viability of murine splenocytes and inhibited proliferation of MCF-7 breast cancer cells and HepG2 hepatoma cells with an IC₅₀ of 0.21, 0.97, and 1.37 μM, respectively. It impeded mycelial growth in the fungi Rhizoctonia solani with an IC₅₀ of 39 μM but there was no effect on a variety of other filamentous fungi, including Fusarium oxysporum, Helminthosporium maydis, Valsa mali and Mycosphaerella arachidicola. Lebbeckalysin inhibited growth of Escherichia coli with an IC₅₀ of 0.52 μM.

MeSH terms

  • Albizzia / chemistry*
  • Animals
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology*
  • Antifungal Agents / isolation & purification
  • Antifungal Agents / metabolism
  • Antifungal Agents / pharmacology*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Escherichia coli / drug effects
  • Fungi / drug effects
  • Hemolysin Proteins / isolation & purification
  • Hemolysin Proteins / metabolism
  • Hemolysin Proteins / pharmacology*
  • Humans
  • Hydrogen-Ion Concentration
  • Inhibitory Concentration 50
  • Mice
  • Mice, Inbred C57BL
  • Microbial Sensitivity Tests
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology
  • Rabbits
  • Seeds / chemistry
  • Temperature


  • Anti-Bacterial Agents
  • Antifungal Agents
  • Hemolysin Proteins
  • Plant Extracts