Anti-inflammatory effect of hydrogen-rich saline in a rat model of regional myocardial ischemia and reperfusion

Int J Cardiol. 2011 Apr 1;148(1):91-5. doi: 10.1016/j.ijcard.2010.08.058. Epub 2010 Sep 20.


Introduction: To study the possible anti-inflammatory effect of hydrogen-rich saline (H(2) saline) on rat hearts with regional myocardial ischemia and reperfusion (I/R).

Methods: Sixty-six rats were equally randomized to three groups: sham-operated group, I/R group (control group) and I/R plus H(2) saline treatment group. Myocardial I/R was established by occlusion of the left anterior descending (LAD) coronary artery for 30 min and reperfusion for 24 h.

Results: H(2) saline treatment attenuated I/R-induced cardiac cell apoptosis, presenting as significant improvement of heart function parameters 24h after reperfusion, including left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVEDP), +(dP/dt)max and -(dP/dt)max. It also decreased neutrophil infiltration, 3-nitrotyrosine level, expression of intercellular adhesion molecule 1(ICAM-1) and myeloperoxidase (MPO) activity in the area at risk zones (AAR) of rat hearts subjected to regional myocardial I/R, and attenuated the increase of I/R induced proinflammatory cytokine tumor necrosis factor-α (TNF-a) and interleukin-1β (IL-1b) levels in the AAR.

Conclusion: H(2) saline has an anti-inflammatory effect on rat hearts with regional myocardial I/R.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / administration & dosage*
  • Cardiotonic Agents / administration & dosage
  • Disease Models, Animal*
  • Hydrogen / administration & dosage*
  • Male
  • Myocardial Ischemia / drug therapy*
  • Myocardial Ischemia / metabolism
  • Myocardial Ischemia / pathology*
  • Myocardial Reperfusion / methods*
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Chloride / administration & dosage*


  • Anti-Inflammatory Agents, Non-Steroidal
  • Cardiotonic Agents
  • Sodium Chloride
  • Hydrogen