Stable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species

Appl Environ Microbiol. 2010 Nov;76(22):7635-40. doi: 10.1128/AEM.01188-10. Epub 2010 Sep 17.

Abstract

Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Burkholderia / genetics*
  • Cloning, Molecular / methods
  • DNA Transposable Elements
  • Fluorescence*
  • Genetic Vectors*
  • Genetics, Microbial / methods
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Molecular Biology / methods
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Staining and Labeling / methods*

Substances

  • DNA Transposable Elements
  • Luminescent Proteins
  • Recombinant Fusion Proteins