Novel Activity of Rhodobacter Sphaeroides Spheroidene Monooxygenase CrtA Expressed in Escherichia Coli

Appl Environ Microbiol. 2010 Nov;76(21):7328-31. doi: 10.1128/AEM.01606-10. Epub 2010 Sep 17.

Abstract

The spheroidene monooxygenase CrtA of Rhodobacter sphaeroides introduces a keto group and/or hydroxy group at the ends of nonnative substrates in Escherichia coli, resulting in the production of novel oxocarotenoids. The heme-containing CrtA is not a P450 enzyme but a new type of oxygenase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carotenoids / isolation & purification
  • Carotenoids / metabolism
  • Chromatography, Thin Layer
  • Crystallography, X-Ray
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Mixed Function Oxygenases / genetics*
  • Mixed Function Oxygenases / metabolism
  • Organisms, Genetically Modified / genetics
  • Organisms, Genetically Modified / metabolism
  • Rhodobacter sphaeroides / enzymology*
  • Rhodobacter sphaeroides / genetics

Substances

  • spheroidene
  • Carotenoids
  • Mixed Function Oxygenases