Tomato paste rich in lycopene protects against cutaneous photodamage in humans in vivo: a randomized controlled trial

Br J Dermatol. 2011 Jan;164(1):154-62. doi: 10.1111/j.1365-2133.2010.10057.x. Epub 2010 Nov 29.

Abstract

Background: Previous epidemiological, animal and human data report that lycopene has a protective effect against ultraviolet radiation (UVR)-induced erythema.

Objectives: We examined whether tomato paste--rich in lycopene, a powerful antioxidant--can protect human skin against UVR-induced effects partially mediated by oxidative stress, i.e. erythema, matrix changes and mitochondrial DNA (mtDNA) damage.

Methods: In a randomized controlled study, 20 healthy women (median age 33 years, range 21-47; phototype I/II) ingested 55 g tomato paste (16 mg lycopene) in olive oil, or olive oil alone, daily for 12 weeks. Pre- and postsupplementation, UVR erythemal sensitivity was assessed visually as the minimal erythema dose (MED) and quantified with a reflectance instrument. Biopsies were taken from unexposed and UVR-exposed (3 × MED 24 h earlier) buttock skin pre- and postsupplementation, and analysed immunohistochemically for procollagen (pC) I, fibrillin-1 and matrix metalloproteinase (MMP)-1, and by quantitative polymerase chain reaction for mtDNA 3895-bp deletion.

Results: Mean ± SD erythemal D(30) was significantly higher following tomato paste vs. control (baseline, 26·5 ± 7·5 mJ cm(-2); control, 23 ± 6·6 mJ cm(-2); tomato paste, 36·6 ± 14·7 mJ cm(-2); P = 0·03), while the MED was not significantly different between groups (baseline, 35·1 ± 9·9 mJ cm(-2); control, 32·6 ± 9·6 mJ cm(-2); tomato paste, 42·2 ± 11·3 mJ cm(-2)). Presupplementation, UVR induced an increase in MMP-1 (P = 0·01) and a reduction in fibrillin-1 (P = 0·03). Postsupplementation, UVR-induced MMP-1 was reduced in the tomato paste vs. control group (P = 0·04), while the UVR-induced reduction in fibrillin-1 was similarly abrogated in both groups, and an increase in pCI deposition was seen following tomato paste (P = 0·05). mtDNA 3895-bp deletion following 3 × MED UVR was significantly reduced postsupplementation with tomato paste (P = 0·01).

Conclusions: Tomato paste containing lycopene provides protection against acute and potentially longer-term aspects of photodamage.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antioxidants / administration & dosage
  • Biopsy
  • Buttocks
  • Carotenoids / administration & dosage*
  • DNA Damage / genetics
  • DNA, Mitochondrial / genetics
  • Dietary Supplements
  • Dose-Response Relationship, Radiation
  • Erythema / etiology
  • Erythema / metabolism
  • Erythema / prevention & control*
  • Female
  • Fibrillin-1
  • Fibrillins
  • Humans
  • Immunohistochemistry
  • Lycopene
  • Lycopersicon esculentum*
  • Matrix Metalloproteinase 1 / metabolism
  • Microfilament Proteins / metabolism
  • Middle Aged
  • Plant Preparations / administration & dosage*
  • Polymerase Chain Reaction / methods
  • Procollagen / metabolism
  • Sequence Deletion
  • Skin / metabolism
  • Skin / radiation effects*
  • Ultraviolet Rays / adverse effects*
  • Young Adult

Substances

  • Antioxidants
  • DNA, Mitochondrial
  • FBN1 protein, human
  • Fibrillin-1
  • Fibrillins
  • Microfilament Proteins
  • Plant Preparations
  • Procollagen
  • Carotenoids
  • Matrix Metalloproteinase 1
  • Lycopene