Novel markers in zero-hour kidney biopsies indicate graft quality and clinical outcome

Transplantation. 2010 Nov 15;90(9):958-65. doi: 10.1097/TP.0b013e3181f546e8.


Background: In renal transplantation, allograft biopsies provide valuable diagnostic information indicating adverse allograft outcome posttransplantation. To uncover novel candidate markers characteristic of subtle inflammation and immune activation present during the intraoperative period, we investigated messenger RNA (mRNA) gene expression profiles in renal zero biopsies.

Methods: Transcription profiles from deceased donors (n=63) and living donors (n=26) were investigated for inflammation-associated markers in zero-hour biopsies by real-time reverse-transcriptase polymerase chain reaction.

Results: We observed a significant induction of the chemokine receptor 7 ligands [C-C motif] ligand 19/21 in the deceased donor group (P<0.001). Additionally, along with the induction of the activation marker CD69 (P<0.01), we further detected significant elevated mRNA levels of the inducible immunoproteasome subunits PSMB8, PSMB9, and PSMB10 (P<0.001, respectively). Candidate markers were further tested for posttransplantation clinical outcomes showing the potential to predict the development of delayed graft function, acute rejection, and renal function after 6 months. For instance, by combining mRNA gene expression profiles with clinical patient data, the analysis revealed high sensitivity (95%) and specificity (84%, area under the curve=0.93) for the prediction of acute rejection.

Conclusions: Zero-hour biopsies of renal allografts may provide useful information on subclinical pathological changes in the grafted kidney. The identification of CCL19/21 or PSMB8/9/10 makes these molecules particularly suitable as potential candidate targets for therapeutic interventions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD / genetics
  • Biopsy
  • Cadaver
  • Chemokine CCL19 / genetics
  • Chemokine CCL21 / genetics
  • Delayed Graft Function / immunology
  • Delayed Graft Function / pathology
  • Gene Expression Regulation
  • Humans
  • Intercellular Adhesion Molecule-1 / genetics
  • Interleukin-15 / genetics
  • Kidney / immunology
  • Kidney / pathology*
  • Kidney Transplantation / immunology
  • Kidney Transplantation / physiology*
  • Living Donors
  • Proteasome Endopeptidase Complex / genetics
  • RNA, Messenger / genetics
  • Reoperation
  • Tissue Donors
  • Transplantation, Homologous
  • Treatment Outcome


  • Antigens, CD
  • Chemokine CCL19
  • Chemokine CCL21
  • Interleukin-15
  • RNA, Messenger
  • Intercellular Adhesion Molecule-1
  • Proteasome Endopeptidase Complex