Bionanoconjugate-based composites for decontamination of nerve agents

Biotechnol Prog. Nov-Dec 2010;26(6):1622-8. doi: 10.1002/btpr.498. Epub 2010 Sep 21.


We have developed enzyme-based composites that rapidly and effectively detoxify simulants of V- and G-type chemical warfare nerve agents. The approach was based on the efficient immobilization of organophosphorus hydrolase onto carbon nanotubes to form active and stable conjugates that were easily entrapped in commercially available paints. The resulting catalytic-based composites showed no enzyme leaching and rendered >99% decontamination of 10 g/m(2) paraoxon, a simulant of the V-type nerve agent, in 30 minutes and >95% decontamination of diisopropylfluorophosphate, a simulant of G-type nerve agent, in 45 minutes. The formulations are expected to be environmentally friendly and to offer an easy to use, on demand, decontamination alternative to chemical approaches for sustainable material self-decontamination.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aryldialkylphosphatase / chemistry
  • Aryldialkylphosphatase / metabolism*
  • Biocatalysis
  • Chemical Warfare Agents / chemistry
  • Chemical Warfare Agents / metabolism*
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism*
  • Nanoconjugates / chemistry*
  • Nanotubes, Carbon / chemistry*
  • Oxidation-Reduction


  • Chemical Warfare Agents
  • Enzymes, Immobilized
  • Nanoconjugates
  • Nanotubes, Carbon
  • Aryldialkylphosphatase