Isolation and characterization of equine amniotic fluid-derived multipotent stem cells

Cytotherapy. 2011 Mar;13(3):341-9. doi: 10.3109/14653249.2010.520312. Epub 2010 Sep 22.


Background aims: Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equine AF stem cells. We have isolated equine AF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation.

Methods: AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll-Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation assay was conducted.

Results: eAF-MSC could be isolated and the proliferation level was high. eAF-MSC presented typical MSC phenotypic markers, as determined by flow cytometry. Moreover, eAF-MSC showed a trilineage differentiation capability.

Conclusions: Equine AF is a good source of MSC. Furthermore, eAF-MSC may be useful as a cell therapy application for horses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipogenesis
  • Amniotic Fluid / cytology*
  • Animals
  • Cell Lineage
  • Cell Proliferation
  • Cell Separation / methods*
  • Cells, Cultured
  • Chondrogenesis
  • Flow Cytometry
  • Horses
  • Humans
  • Immunophenotyping
  • Multipotent Stem Cells / cytology*
  • Multipotent Stem Cells / metabolism
  • Osteogenesis
  • Sequence Homology, Amino Acid