Investigation of MADS domain transcription factor dynamics in the floral meristem

Plant Signal Behav. 2010 Oct;5(10):1260-2. doi: 10.4161/psb.5.10.12949. Epub 2010 Oct 1.

Abstract

To study the importance of intercellular transport for MADS domain transcription factor functioning during floral development, we analyzed the dynamic behavior of fluorescently-tagged MADS domain proteins in transgenic plants by Confocal Laser Scanning Microscopy. These analyses, described in a recent paper in The Plant Journal, provided proof for previous suggestions that the Arabidopsis thaliana C-type protein AGAMOUS has a non-cell-autonomous role in floral meristem integrity. Furthermore, it indicated a possible non-cell-autonomous role for the B-type proteins APETALA3 and PISTILLATA, and the E-type protein SEPALLATA3, through lateral intercellular movement in the floral meristem. In this addendum we compare some of the available fluorescent protein-based technologies for the investigation of transcription factor movements and dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis / radiation effects
  • Arabidopsis Proteins / metabolism*
  • Flowers / metabolism*
  • Flowers / radiation effects
  • Fluorescence Recovery After Photobleaching
  • Fluorescent Dyes / metabolism
  • Light
  • MADS Domain Proteins / metabolism*
  • Meristem / metabolism*
  • Meristem / radiation effects

Substances

  • AP1 protein, Arabidopsis
  • Arabidopsis Proteins
  • Fluorescent Dyes
  • MADS Domain Proteins