Tetrazine-trans-cyclooctene ligation for the rapid construction of 18F labeled probes

Chem Commun (Camb). 2010 Nov 14;46(42):8043-5. doi: 10.1039/c0cc03078c. Epub 2010 Sep 22.


A radiolabeling method for bioconjugation based on the Diels-Alder reaction between 3,6-diaryl-s-tetrazines and an (18)F-labeled trans-cyclooctene is described. The reaction proceeds with exceptionally fast rates, making it an effective conjugation method within seconds at low micromolar concentrations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Cyclooctanes / chemistry*
  • Fluorine Radioisotopes / chemistry*
  • Radiometry


  • Cyclooctanes
  • Fluorine Radioisotopes