Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth

Virology. 2010 Nov 25;407(2):360-7. doi: 10.1016/j.virol.2010.08.018. Epub 2010 Sep 21.

Abstract

Human herpesvirus 6 (HHV-6) glycoprotein Q1 (gQ1), a unique gene in HHV-6, forms a complex with glycoproteinH (gH) and gL, which is the viral ligand for its cellular receptor, CD46. However, whether gQ1 is essential for virus growth is unknown, because a system is lacking for making gene knockouts for HHV-6. Recently, bacterial artificial chromosome (BAC) and E. coli mutagenesis techniques have been applied to herpesvirus investigation. Here we successfully inserted the HHV-6A genome into a BAC, and obtained reconstituted infectious virus from the HHV-6A-containing BAC DNA. Using this system, we generated a gQ1 mutant virus genome, which failed to yield reconstituted infectious virus, whereas its revertant virus could be produced, indicating that the HHV-6 gQ1 gene is essential for virus growth. Therefore, we successfully applied BAC and E. coli mutagenesis techniques to the study of HHV-6, and discovered that HHV-6 gQ1 is an essential gene for virus growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Chromosomes, Artificial, Bacterial
  • Escherichia coli / genetics
  • Gene Deletion
  • Genes, Essential
  • Genes, Viral
  • Glycoproteins / genetics*
  • Glycoproteins / metabolism
  • Herpesvirus 6, Human / genetics
  • Herpesvirus 6, Human / growth & development*
  • Herpesvirus 6, Human / metabolism
  • Humans
  • Ligands
  • Mutagenesis
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / metabolism

Substances

  • Glycoproteins
  • Ligands
  • Viral Envelope Proteins