Non-contact addition, metering, and distribution of liquids into centrifugal microfluidic devices in motion

Anal Chem. 2010 Oct 15;82(20):8386-9. doi: 10.1021/ac1024478.


A three-dimensional structure was constructed to demonstrate contact-free liquid addition and distribution to a conventional centrifugal microfluidic platform. The structure allowed a continuous stream of liquid to be added to the rotating platform where it was dispensed and metered to a series of reservoirs with 9% relative standard deviation of the volume. An off-platform generated gradient was used to demonstrate this design's potential to make gradient centrifugal microfluidic chromatography possible without the use of platform space for liquid storage. The system demonstrates a convenient and versatile "World-to-Chip" or more appropriately, World-to-Disk, macro-to-micro interface.