Cytotoxic effects of activated alveolar macrophages on lung carcinoma cells via cell-to-cell contact and nitric oxide

Anticancer Res. 2010 Aug;30(8):3135-41.

Abstract

Aim: It is possible that macrophages may be effective for cancer treatment because once activated, lung macrophages have enhanced contact with lung tumor cells and have a cytotoxic effect. In this paper, we report that nitric oxide (NO) produced by lung macrophages activated with lipopolysaccharide (LPS) suppressed cell growth of human lung adenocarcinoma cells.

Materials and methods: A549, a lung adenocarcinoma cell line, was cultured with NR8383, a rat alveolar macrophage cell line, in the presence and absence of LPS. The effect of LPS on the growth rate of A549 cells was examined as a function of NO production under cell-to-cell contact conditions.

Results: NR8383 cells showed potent cytostatic and cytocidal effects on A549 cells when both cells were co-cultured in the presence of LPS. These effects were mainly due to the production of NO, but another possible mechanism, such as cell-to-cell contact, may also be involved.

Conclusion: Activation of alveolar macrophages by LPS suppresses the growth of lung carcinoma cells via NO production under cell-to-cell contact conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology*
  • Animals
  • Cell Communication*
  • Cell Division
  • Cell Line, Tumor
  • Coculture Techniques
  • Humans
  • Lipopolysaccharides / pharmacology
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology*
  • Macrophage Activation*
  • Macrophages, Alveolar / immunology*
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / biosynthesis*
  • Rats

Substances

  • Lipopolysaccharides
  • Nitric Oxide