Traditional mould analysis compared to a DNA-based method of mould analysis

Crit Rev Microbiol. 2011 Feb;37(1):15-24. doi: 10.3109/1040841X.2010.506177. Epub 2010 Sep 29.

Abstract

Traditional environmental mould analysis is based on microscopic observations and counting of mould structures collected from the air on a sticky surface or culturing of moulds on growth media for identification and quantification. These approaches have significant limitations. A DNA-based method of mould analysis called mould specific quantitative PCR (MSQPCR) was created for more than 100 moulds. Based on a national sampling and analysis by MSQPCR of dust in US homes, a scale for comparing the mould burden in homes was created called the Environmental Relative Mouldiness Index (ERMI).

Publication types

  • Review

MeSH terms

  • DNA, Fungal / analysis
  • DNA, Fungal / genetics
  • Environmental Microbiology*
  • Fungi / genetics
  • Fungi / isolation & purification*
  • Housing
  • Polymerase Chain Reaction / methods*

Substances

  • DNA, Fungal