Genome-wide model for the normal eukaryotic DNA replication fork

Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17674-9. doi: 10.1073/pnas.1010178107. Epub 2010 Sep 27.


To investigate DNA replication enzymology across the nuclear genome of budding yeast, deep sequencing was used to establish the pattern of uncorrected replication errors generated by an asymmetric mutator variant of DNA polymerase δ (Pol δ). Sequencing of 16 genomes identified 1,206-bp substitutions generated over 33 generations by L612M Pol δ in a mismatch repair defective strain. Alignment of sequences flanking these substitutions identified "hotspot" motifs for Pol δ replication errors. The substitutions were distributed evenly across all 16 chromosomes. The vast majority were transitions that occurred with a strand bias that varied in a predictable manner relative to known functional origins of replication. This strand bias strongly supports the idea that Pol δ is primarily a lagging strand polymerase during replication across the entire nuclear genome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pair Mismatch / genetics
  • Base Sequence
  • DNA Polymerase III / genetics*
  • DNA Replication / genetics*
  • DNA Replication / physiology
  • Gene Library
  • Genome, Fungal / genetics
  • Models, Genetic*
  • Molecular Sequence Data
  • Mutation / genetics*
  • Polymorphism, Single Nucleotide / genetics
  • Replication Origin / genetics
  • Saccharomyces cerevisiae / genetics*
  • Sequence Alignment
  • Sequence Analysis, DNA


  • DNA Polymerase III