Lab-on-a-chip flow cytometer employing color-space-time coding

Appl Phys Lett. 2010 Aug 30;97(9):093704. doi: 10.1063/1.3481695. Epub 2010 Sep 2.


We describe a fluorescent detection technique for a lab-on-a-chip flow cytometer. Fluorescent emission is encoded into a time-dependent signal as a fluorescent cell or bead traverses a waveguide array with integrated spatial filters and color filters. Different from conventional colored filters with well-defined transmission spectral window, the integrated color filters are designed to have broad transmission characteristics, similar to the red-green-blue photoreceptors in the retina of human eye. This unique design allows us to detect multiple fluorescent colors with only three color filters based on the technique of color-space-time coding using only one single photomultiplier tube or avalanche photodetector.