Interferon-gamma +874 T/A and interleukin-10 -1082 A/G single nucleotide polymorphism in Egyptian children with tuberculosis

Scand J Immunol. 2010 Oct;72(4):358-64. doi: 10.1111/j.1365-3083.2010.02426.x.


The aim was to investigate the association of interferon-gamma (IFN-γ) +874 T/A and interleukin-10 (IL-10)-1082 A/G single nucleotide polymorphisms with tuberculous infection and post-BCG lymphadenitis in Egyptian children. IFN-γ +874 T/A and IL-10 -1082 A/G polymorphism detection by amplification refractory mutation system technique was carried out for 110 patients with TB, 40 patients with post-BCG lymphadenitis and 118 healthy controls. IFN-γ +874 A allele was higher in TB and post-BCG patients than those in healthy controls (Pc=0.006 and 0.002, respectively). IFN-γ +874 genotype AA was significantly higher in patients with TB than that in control (Pc=0.015), in extrapulmonary than patients with pulmonary TB (PTB) (Pc=0.009), and young children with TB below 5 years (Pc=0.024). No statistically significant differences were observed between patients with TB and controls for the frequency of IL-10(-1082) alleles or genotypes (P>0.05); however, a statistically significant difference in the frequency of IL-10 (-1082) GG genotype was found between patients with pulmonary and extrapulmonary TB (Pc=0.003). Low producer IFN-γ +874 A/A genotype is associated with post-BCG lymphadenitis and TB disease especially in younger children below 5 years. IL-10-1082 G/G genotype did not exhibit significant association except for increased GG frequency in PTB. Both cytokine polymorphisms have no relation to tuberculin reaction in patients with TB.

MeSH terms

  • Adolescent
  • BCG Vaccine / administration & dosage
  • BCG Vaccine / adverse effects
  • BCG Vaccine / immunology
  • Child
  • Child, Preschool
  • Egypt
  • Female
  • Gene Frequency
  • Genotype
  • Humans
  • Infant
  • Interferon-gamma / genetics*
  • Interleukin-10 / genetics*
  • Lymphadenitis / chemically induced
  • Lymphadenitis / immunology
  • Male
  • Odds Ratio
  • Polymorphism, Single Nucleotide*
  • Tuberculosis / genetics*
  • Tuberculosis / immunology
  • Tuberculosis / prevention & control
  • Tuberculosis, Pulmonary / genetics
  • Tuberculosis, Pulmonary / immunology
  • Tuberculosis, Pulmonary / prevention & control


  • BCG Vaccine
  • Interleukin-10
  • Interferon-gamma