Abstract
Hesperidin is a naturally occurring flavonoid that has been reported to possess anticancer effects. The purpose of this study is to evaluate the effect of hesperidin in modulating the expressions of cyclooxygenase-2 (COX-2), mast cells (MCs) and matrix metalloproteinases (MMPs) during benzo(a)pyrene (B(a)P) induced lung carcinogenesis in mice. B(a)P (50 mg/kg body weight) induced animals showed increased mast cell density (MCD) as revealed by toluidine blue staining and severe expression of COX-2 along with upregulated expression of MMP-2 and MMP-9 as revealed by Western blotting and immunohistochemistry. Supplementation of hesperidin (25 mg/kg body weight) to lung cancer bearing mice attenuated MCD and downregulated the expressions of COX-2, MMP-2 and MMP-9. These observations show that hesperidin exerts its anti-carcinogenic activity against lung cancer by altering the expressions of COX-2, MMP-2 and MMP-9.
Copyright © 2010 Elsevier B.V. All rights reserved.
MeSH terms
-
Animals
-
Anticarcinogenic Agents / adverse effects
-
Anticarcinogenic Agents / therapeutic use*
-
Antineoplastic Agents, Phytogenic / adverse effects
-
Antineoplastic Agents, Phytogenic / therapeutic use*
-
Benzo(a)pyrene / toxicity
-
Blotting, Western
-
Carcinogens / toxicity
-
Cell Count
-
Cyclooxygenase 2 / metabolism*
-
Dietary Supplements
-
Down-Regulation / drug effects
-
Hesperidin / adverse effects
-
Hesperidin / therapeutic use*
-
Immunohistochemistry
-
Lung / drug effects
-
Lung / metabolism
-
Lung / pathology
-
Lung Neoplasms / drug therapy*
-
Lung Neoplasms / metabolism
-
Lung Neoplasms / pathology
-
Lung Neoplasms / prevention & control*
-
Male
-
Mast Cells / drug effects
-
Mast Cells / pathology
-
Matrix Metalloproteinase 2 / metabolism
-
Matrix Metalloproteinase 9 / metabolism
-
Matrix Metalloproteinases, Secreted / metabolism*
-
Mice
Substances
-
Anticarcinogenic Agents
-
Antineoplastic Agents, Phytogenic
-
Carcinogens
-
Benzo(a)pyrene
-
Hesperidin
-
Ptgs2 protein, mouse
-
Cyclooxygenase 2
-
Matrix Metalloproteinases, Secreted
-
Matrix Metalloproteinase 2
-
Mmp2 protein, mouse
-
Matrix Metalloproteinase 9
-
Mmp9 protein, mouse