A family of plasmodesmal proteins with receptor-like properties for plant viral movement proteins

PLoS Pathog. 2010 Sep 23;6(9):e1001119. doi: 10.1371/journal.ppat.1001119.

Abstract

Plasmodesmata (PD) are essential but poorly understood structures in plant cell walls that provide symplastic continuity and intercellular communication pathways between adjacent cells and thus play fundamental roles in development and pathogenesis. Viruses encode movement proteins (MPs) that modify these tightly regulated pores to facilitate their spread from cell to cell. The most striking of these modifications is observed for groups of viruses whose MPs form tubules that assemble in PDs and through which virions are transported to neighbouring cells. The nature of the molecular interactions between viral MPs and PD components and their role in viral movement has remained essentially unknown. Here, we show that the family of PD-located proteins (PDLPs) promotes the movement of viruses that use tubule-guided movement by interacting redundantly with tubule-forming MPs within PDs. Genetic disruption of this interaction leads to reduced tubule formation, delayed infection and attenuated symptoms. Our results implicate PDLPs as PD proteins with receptor-like properties involved the assembly of viral MPs into tubules to promote viral movement.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / growth & development
  • Arabidopsis / metabolism
  • Arabidopsis / virology
  • Cell Communication
  • Cell Wall / metabolism
  • Chenopodium quinoa / growth & development
  • Chenopodium quinoa / metabolism
  • Chenopodium quinoa / virology
  • Immunoblotting
  • Plant Diseases / virology*
  • Plant Leaves / growth & development
  • Plant Leaves / metabolism
  • Plant Leaves / virology
  • Plant Viral Movement Proteins / metabolism*
  • Plant Viruses / physiology*
  • Plasmodesmata / metabolism*
  • Plasmodesmata / virology*
  • Protein Transport
  • RNA, Viral / genetics
  • Receptors, Cell Surface / metabolism*
  • Tobacco / growth & development
  • Tobacco / metabolism
  • Tobacco / virology

Substances

  • Plant Viral Movement Proteins
  • RNA, Viral
  • Receptors, Cell Surface