Comprehensive Proteomics

Curr Opin Biotechnol. 2011 Feb;22(1):3-8. doi: 10.1016/j.copbio.2010.09.002. Epub 2010 Oct 1.

Abstract

Extensive proteome discovery projects using a variety of mass spectrometric techniques have identified proteins matching to 50-70% of the predicted gene models of various species. Comprehensive proteome coverage is desirable for the unbiased comparison of protein quantities between different biological states and for the meaningful comparison of data from multiple samples. Here we discuss the feasibility of this goal in the light of recent technological developments.

Publication types

  • Review

MeSH terms

  • Animals
  • Chromatography, Liquid / instrumentation
  • Chromatography, Liquid / methods
  • Gene Expression
  • Humans
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods
  • Mathematical Computing
  • Models, Biological
  • Peptide Fragments / analysis*
  • Proteins / chemistry
  • Proteins / genetics
  • Proteome / analysis*
  • Proteome / chemistry
  • Proteomics* / methods

Substances

  • Peptide Fragments
  • Proteins
  • Proteome