Doublecortin (DCX) immunoreactivity in hippocampus of chronic refractory temporal lobe epilepsy patients with hippocampal sclerosis

Seizure. 2010 Nov;19(9):567-72. doi: 10.1016/j.seizure.2010.09.004.


Introduction: Status epilepticus increases the production of new neurons (hippocampal neurogenesis) and promotes aberrant migration. However chronic experimental models of epilepsy and studies performed in human epilepsy showed controversial results suggesting a reduction in hippocampal neurogenesis in late stages of the disease. Doublecortin (DCX) has been validated to determine alterations in the production of new neurons in the human hippocampus.

Objectives: Determine DCX expression in human hippocampal sclerosis (HS) from patients who underwent epilepsy surgery for refractory temporal lobe epilepsy (TLE).

Methods: Hippocampal sections of 9 patients with HS and TLE who underwent surgery, were processed using immunoperoxidase for DCX. Archival material from 5 normal post-mortem hippocampus were simultaneously processed.

Results: Significantly lower staining intensity was observed in DCX-positive neurons localized in dentate gyrus (DG) and in CA1 of epileptic hippocampus; lower DCX reactive area was observed in pyramidal layers of CA1; and a reduced in the mean number of DCX-positive neurons were determined in DG compared to normal hippocampus (p<0.05).

Conclusions: This study found a decrease in DCX expression in hippocampus of patients with HS and chronic and refractory TLE suggesting alterations in NG and hippocampal synaptogenesis with potential cognitive and emotional repercussion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Dentate Gyrus / pathology
  • Doublecortin Domain Proteins
  • Doublecortin Protein
  • Epilepsy, Temporal Lobe / drug therapy
  • Epilepsy, Temporal Lobe / immunology
  • Epilepsy, Temporal Lobe / pathology*
  • Epilepsy, Temporal Lobe / surgery
  • Female
  • Hippocampus / immunology
  • Hippocampus / pathology*
  • Hippocampus / physiopathology
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Microtubule-Associated Proteins / immunology*
  • Microtubule-Associated Proteins / physiology
  • Neuropeptides / immunology*
  • Neuropeptides / physiology
  • Sclerosis


  • DCX protein, human
  • Doublecortin Domain Proteins
  • Doublecortin Protein
  • Microtubule-Associated Proteins
  • Neuropeptides