Ottowia pentelensis sp. nov., a floc-forming betaproteobacterium isolated from an activated sludge system treating coke plant effluent

Int J Syst Evol Microbiol. 2011 Sep;61(Pt 9):2146-2150. doi: 10.1099/ijs.0.020818-0. Epub 2010 Oct 1.

Abstract

A Gram-negative-staining, short-rod-shaped, floc-forming bacterium, designated strain RB3-7(T), was isolated from a laboratory-scale activated sludge system treating coke plant effluent. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate was distantly related (≤ 95.8 % similarity) to Ottowia thiooxydans K11(T) within the family Comamonadaceae. Strain RB3-7(T) was catalase- and oxidase-positive and non-motile. The predominant fatty acids were C₁₆:₀, cyclo C₁₇:₀, C₁₈:₁ω7c and C₁₆:₁ω7c, and the major respiratory quinone was Q-8. The G+C content of the genomic DNA of strain RB3-7(T) was 68.5 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain RB3-7(T) is considered to represent a novel species of the genus Ottowia, for which the name Ottowia pentelensis sp. nov. is proposed. The type strain is RB3-7(T) ( = DSM 21699(T) = NCAIM B 02336(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Catalase / metabolism
  • Cluster Analysis
  • Comamonadaceae / classification*
  • Comamonadaceae / genetics
  • Comamonadaceae / isolation & purification*
  • Comamonadaceae / physiology
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Molecular Sequence Data
  • Oxidoreductases / metabolism
  • Phylogeny
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sewage / microbiology*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Quinones
  • RNA, Ribosomal, 16S
  • Sewage
  • Oxidoreductases
  • Catalase

Associated data

  • GENBANK/EU518930