Structure of the Dom34-Hbs1 complex and implications for no-go decay

Nat Struct Mol Biol. 2010 Oct;17(10):1233-40. doi: 10.1038/nsmb.1922. Epub 2010 Oct 3.


No-go decay (NGD) targets mRNAs with stalls in translation elongation for endonucleolytic cleavage in a process involving the Dom34 and Hbs1 proteins. The crystal structure of a Schizosaccharomyces pombe Dom34-Hbs1 complex reveals an overall shape similar to that of eRF1-eRF3-GTP and EF-Tu-tRNA-GDPNP. Similarly to eRF1 and GTP binding to eRF3, Dom34 and GTP bind to Hbs1 with strong cooperativity, and Dom34 acts as a GTP-dissociation inhibitor (GDI). A marked conformational change in Dom34 occurs upon binding to Hbs1, leading Dom34 to resemble a portion of a tRNA and to position a conserved basic region in a position expected to be near the peptidyl transferase center. These results support the idea that the Dom34-Hbs1 complex functions to terminate translation and thereby commit mRNAs to NGD. Consistent with this role, NGD at runs of arginine codons, which cause a strong block to elongation, is independent of the Dom34-Hbs1 complex.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calorimetry
  • Crystallography, X-Ray
  • Endoribonucleases / metabolism
  • Guanosine Triphosphate / metabolism
  • Models, Molecular
  • Peptide Elongation Factor Tu / chemistry
  • Protein Binding
  • Protein Biosynthesis
  • Protein Conformation
  • Protein Interaction Mapping
  • Protein Structure, Tertiary
  • RNA Stability / physiology*
  • RNA, Fungal / metabolism*
  • RNA, Messenger / metabolism*
  • RNA, Transfer / chemistry
  • Recombinant Fusion Proteins / chemistry
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces pombe Proteins
  • Structure-Activity Relationship
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / physiology


  • RNA, Fungal
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • Guanosine Triphosphate
  • RNA, Transfer
  • Endoribonucleases
  • Peptide Elongation Factor Tu

Associated data

  • PDB/3MCA.