The molecular principles of N-end rule recognition

Nat Struct Mol Biol. 2010 Oct;17(10):1164-5. doi: 10.1038/nsmb1010-1164.


The N-end rule pathway is a proteolytic system in which recognition components (N-recognins) recognize a set of N-terminal residues as part of degradation signals (N-degrons). Two studies in this issue report the structures of Ubr boxes, a substrate recognition domain of eukaryotic N-recognins. We discuss how eukaryotic and prokaryotic N-recognins use a similar molecular principle to recognize a different set of N-degrons.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Comment

MeSH terms

  • Acetylation
  • Amino Acids / chemistry
  • Animals
  • Carrier Proteins / physiology
  • Escherichia coli Proteins / physiology
  • Eukaryotic Cells / metabolism
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Models, Molecular
  • Peptide Hydrolases / metabolism*
  • Prokaryotic Cells / metabolism
  • Protein Binding
  • Protein Conformation
  • Protein Processing, Post-Translational
  • Protein Structure, Tertiary
  • Saccharomyces cerevisiae Proteins / metabolism
  • Structure-Activity Relationship
  • Substrate Specificity
  • Ubiquitin-Protein Ligases / metabolism*
  • Zinc Fingers / physiology


  • Amino Acids
  • Carrier Proteins
  • ClpS protein, E coli
  • Escherichia coli Proteins
  • Saccharomyces cerevisiae Proteins
  • UBR1 protein, S cerevisiae
  • Ubiquitin-Protein Ligases
  • Peptide Hydrolases