To investigate the short-term effects of laser-assisted subepithelial keratectomy (LASEK) on the corneal endothelium, 10 patients undergoing LASEK for myopia without complications (20 eyes) were observed. Each eye was evaluated by slit-lamp biomicroscopy and non-contact specular microscopy pre-operatively, and at 15 min, 1 day and 1 week post-operatively. The decrease in endothelial cell density was statistically significant at 15 min post-operatively, and the difference between pre-operative and 15-min post-operative coefficient of variation of cell size was also statistically significant. The percentage of hexagonal cells was significantly different from baseline levels at 15 min and at 1 day post-operatively. All parameters at 1 week post-operatively were not statistically different from those observed pre-operatively. Immediate changes in endothelial cell number and morphology occurred following LASEK, but endothelial morphology returned to the pre-operative condition after 1 week. Long-term follow-up is needed to identify further consequences of this procedure.