Angiotensin II diminishes the effect of SGK1 on the WNK4-mediated inhibition of ROMK1 channels

Kidney Int. 2011 Feb;79(4):423-31. doi: 10.1038/ki.2010.380. Epub 2010 Oct 6.

Abstract

ROMK1 channels are located in the apical membrane of the connecting tubule and cortical collecting duct and mediate the potassium secretion during normal dietary intake. We used a perforated whole-cell patch clamp to explore the effect of angiotensin II on these channels in HEK293 cells transfected with green fluorescent protein (GFP)-ROMK1. Angiotensin II inhibited ROMK1 channels in a dose-dependent manner, an effect abolished by losartan or by inhibition of protein kinase C. Furthermore, angiotensin II stimulated a protein kinase C-sensitive phosphorylation of tyrosine 416 within c-Src. Inhibition of protein tyrosine kinase attenuated the effect of angiotensin II. Western blot studies suggested that angiotensin II inhibited ROMK1 channels by enhancing its tyrosine phosphorylation, a notion supported by angiotensin II's failure to inhibit potassium channels in cells transfected with the ROMK1 tyrosine mutant (R1Y337A). However, angiotensin II restored the with-no-lysine kinase-4 (WNK4)-induced inhibition of R1Y337A in the presence of serum-glucocorticoids-induced kinase 1 (SGK1), which reversed the inhibitory effect of WNK4 on ROMK1. Moreover, protein tyrosine kinase inhibition abolished the angiotensin II-induced restoration of WNK4-mediated inhibition of ROMK1. Angiotensin II inhibited ROMK channels in the cortical collecting duct of rats on a low sodium diet, an effect blocked by protein tyrosine kinase inhibition. Thus, angiotensin II inhibits ROMK channels by two mechanisms: increasing tyrosine phosphorylation of the channel and synergizing the WNK4-induced inhibition. Hence, angiotensin II may have an important role in suppressing potassium secretion during volume depletion.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Angiotensin II / metabolism
  • Angiotensin II / pharmacology*
  • Animals
  • Diet, Sodium-Restricted
  • HEK293 Cells
  • Humans
  • Immediate-Early Proteins / metabolism*
  • In Vitro Techniques
  • Kidney Tubules, Collecting / drug effects
  • Kidney Tubules, Collecting / metabolism
  • Male
  • Models, Biological
  • Patch-Clamp Techniques
  • Potassium / metabolism
  • Potassium Channels, Inwardly Rectifying / antagonists & inhibitors*
  • Potassium Channels, Inwardly Rectifying / metabolism*
  • Protein-Serine-Threonine Kinases / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • src-Family Kinases / antagonists & inhibitors
  • src-Family Kinases / metabolism

Substances

  • Immediate-Early Proteins
  • KCNJ1 protein, human
  • Kcnj1 protein, rat
  • Potassium Channels, Inwardly Rectifying
  • Angiotensin II
  • src-Family Kinases
  • Protein-Serine-Threonine Kinases
  • WNK4 protein, human
  • serum-glucocorticoid regulated kinase
  • Potassium