Abstract
A series of oligosaccharides (cellobio-oligosaccharides) ranging from degrees of polymer 3 to 6 were synthesized by Leuconostoc mesenteroides B-512 FMCM in the presence of cellobiose. The major oligosaccharides were the trisaccharides, α-D-glucopyranosyl-(1 → 2)-β-D-glucopyranosyl-(1 → 4)-D-glucopyranose and α-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl-(1 → 4)-D-glucopyranose. These cellobio-oligosaccharides were inhibitory on mutansucrase, an enzyme that causes dental caries. They were also found to be effective antifungal agents against Aspergillus terreus acting by inhibiting β-(1 → 3)-glucan synthase, which is required for fungal cell wall formation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antifungal Agents / chemistry
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Antifungal Agents / metabolism*
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Antifungal Agents / pharmacology
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Aspergillus / drug effects
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Aspergillus / enzymology*
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Cellobiose / metabolism*
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / metabolism*
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Enzyme Inhibitors / pharmacology
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Glucosyltransferases / antagonists & inhibitors*
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Glucosyltransferases / metabolism*
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Leuconostoc / chemistry
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Leuconostoc / enzymology*
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Leuconostoc / metabolism
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Molecular Structure
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Oligosaccharides / chemistry
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Oligosaccharides / metabolism*
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Oligosaccharides / pharmacology
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Sucrase / antagonists & inhibitors*
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Sucrase / metabolism
Substances
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Antifungal Agents
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Enzyme Inhibitors
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Oligosaccharides
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Cellobiose
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Glucosyltransferases
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glucan synthase
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dextransucrase
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Sucrase