Direct comparisons of the morphology, migration, cell adhesions, and actin cytoskeleton of fibroblasts in four different three-dimensional extracellular matrices

Tissue Eng Part A. 2011 Mar;17(5-6):713-24. doi: 10.1089/ten.TEA.2010.0273. Epub 2010 Dec 7.

Abstract

Interactions between cells and the extracellular matrix are at the core of tissue engineering and biology. However, most studies of these interactions have used traditional two-dimensional (2D) tissue culture, which is less physiological than three-dimensional (3D) tissue culture. In this study, we compared cell behavior in four types of commonly used extracellular matrix under 2D and 3D conditions. Specifically, we quantified parameters of cell adhesion and migration by human foreskin fibroblasts in cell-derived matrix or hydrogels of collagen type I, fibrin, or basement membrane extract (BME). Fibroblasts in 3D were more spindle shaped with fewer lateral protrusions and substantially reduced actin stress fibers than on 2D matrices; cells failed to spread in 3D BME. Cell-matrix adhesion structures were detected in all matrices. Although the shapes of these cell adhesions differed, the total area per cell occupied by cell-matrix adhesions in 2D and 3D was nearly identical. Fibroblasts migrated most rapidly in cell-derived 3D matrix and collagen and migrated minimally in BME, with highest migration directionality in cell-derived matrix. This identification of quantitative differences in cellular responses to different matrix composition and dimensionality should help guide the development of customized 3D tissue culture and matrix scaffolds for tissue engineering.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Actins / metabolism*
  • Animals
  • Basement Membrane / drug effects
  • Basement Membrane / metabolism
  • Cell Adhesion / drug effects
  • Cell Extracts
  • Cell Movement / drug effects
  • Cell Shape*
  • Collagen / pharmacology
  • Cytoskeleton / drug effects
  • Cytoskeleton / metabolism*
  • Extracellular Matrix / drug effects
  • Extracellular Matrix / metabolism*
  • Fibrin / pharmacology
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Integrin beta1 / metabolism
  • Male
  • Mice
  • Microscopy, Confocal
  • NIH 3T3 Cells
  • Phenotype
  • Vinculin / metabolism

Substances

  • Actins
  • Cell Extracts
  • Integrin beta1
  • Vinculin
  • Fibrin
  • Collagen