Antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans: an in vitro study

Indian J Dent Res. 2010 Jul-Sep;21(3):334-6. doi: 10.4103/0970-9290.70792.


Background: The establishment and maintenance of oral microbiota is related not only to interbacterial coaggregations but also to interactions of these bacteria with yeasts. Hence, it is important for agents used in the treatment of oral diseases to have antifungal properties for effective therapy.

Objective: The main purpose of this study was to evaluate the in vitro antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans.

Materials and methods: The pomegranate peel is separated, dried and powdered. Fennel, cumin and acacia bark obtained from the tree are powdered. Candida is inoculated at 37˚C and seeded on Sabourauds agar medium. Sterilized filter papers saturated with 30 μl of the extracts are placed on the seeded plates and inoculated at 24 and 48 h. Zones of inhibition on all four sides are measured around the filter paper with a vernier caliper. The experiments were repeated on four plates, with four samples of each extract on one plate for all of the extracts.

Results: All the above-mentioned ingredients showed antifungal property, with Punica granatum showing the highest inhibition of Candida albicans with a mean zone of inhibition of 22 mm. P-values <0.05 were obtained for Punica granatum when compared with the other extracts.

Conclusion: The results showed the potential use of these products as cheap and convenient adjuvants to pharmaceutical antifungal products.

Publication types

  • Comparative Study

MeSH terms

  • Acacia*
  • Antifungal Agents / pharmacology*
  • Candida albicans / drug effects*
  • Culture Media
  • Cuminum*
  • Drug Evaluation, Preclinical
  • Foeniculum*
  • Fruit
  • Humans
  • Lythraceae*
  • Mouth / microbiology
  • Mycology / methods
  • Oils, Volatile / pharmacology
  • Plant Bark
  • Plant Extracts / pharmacology*
  • Plant Oils / pharmacology
  • Powders
  • Temperature
  • Time Factors


  • Antifungal Agents
  • Culture Media
  • Oils, Volatile
  • Plant Extracts
  • Plant Oils
  • Powders