Testosterone-lowering activity of canola and hydrogenated soybean oil in the stroke-prone spontaneously hypertensive rat

J Toxicol Sci. 2010 Oct;35(5):743-7. doi: 10.2131/jts.35.743.


Canola and some other types of oil unusually shorten the survival of stroke-prone spontaneously hypertensive rats (SHRSP), compared with soybean oil, perilla oil and animal fats. Since differential effects of canola and soybean oil on steroid hormone metabolism were suggested by a preliminary DNA microarray analysis as a reason for this, the steroid hormone levels in the serum and tissues of SHRSP fed different oils were investigated. The testosterone levels in the serum and the testes were found to be significantly lower in the canola oil group than in the soybean oil group, while no significant differences were detected in the corticosterone and estradiol levels in tissues. In a second experiment, it was found that hydrogenated soybean oil, with a survival-shortening activity comparable to that of canola oil, also decreased the testosterone level in testes to a similar degree. The testosterone-lowering activity of canola and hydrogenated soybean oil observed in SHRSP was considered in relation to other factors possibly affecting the physiology of SHRSP.

Publication types

  • Comparative Study
  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Glands / drug effects
  • Adrenal Glands / metabolism
  • Animals
  • Fatty Acids, Monounsaturated / adverse effects*
  • Female
  • Gene Expression / drug effects
  • Gonadal Steroid Hormones / blood
  • Gonadal Steroid Hormones / genetics
  • Gonadal Steroid Hormones / metabolism
  • Hypertension / blood
  • Hypertension / complications
  • Hypertension / metabolism*
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Ovary / drug effects
  • Ovary / metabolism
  • Prostate / drug effects
  • Prostate / metabolism
  • Rapeseed Oil
  • Rats
  • Rats, Inbred SHR
  • Soybean Oil / adverse effects*
  • Stroke / blood
  • Stroke / complications
  • Stroke / metabolism*
  • Testis / drug effects
  • Testis / metabolism
  • Testosterone / blood
  • Testosterone / genetics
  • Testosterone / metabolism*


  • Fatty Acids, Monounsaturated
  • Gonadal Steroid Hormones
  • Rapeseed Oil
  • Testosterone
  • Soybean Oil