Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1

Nat Med. 2010 Nov;16(11):1295-8. doi: 10.1038/nm.2238. Epub 2010 Oct 10.


Hepatitis C virus (HCV) infection is closely tied to the lipid metabolism of liver cells. Here we identify the triglyceride-synthesizing enzyme diacylglycerol acyltransferase-1 (DGAT1) as a key host factor for HCV infection. DGAT1 interacts with the viral nucleocapsid core and is required for the trafficking of core to lipid droplets. Inhibition of DGAT1 activity or RNAi-mediated knockdown of DGAT1 severely impairs infectious virion production, implicating DGAT1 as a new target for antiviral therapy.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Diacylglycerol O-Acyltransferase / metabolism*
  • HEK293 Cells
  • Hepacivirus / physiology*
  • Humans
  • Immunoprecipitation
  • Transfection
  • Virion / physiology*
  • Virus Assembly / physiology*


  • DGAT1 protein, human
  • Diacylglycerol O-Acyltransferase