Chemical synthesis of the mouse mitochondrial genome

Nat Methods. 2010 Nov;7(11):901-3. doi: 10.1038/nmeth.1515. Epub 2010 Oct 10.


We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

MeSH terms

  • Animals
  • Chromosomes, Artificial, Bacterial
  • DNA, Mitochondrial / chemical synthesis*
  • Genome, Mitochondrial*
  • Mice
  • Polymerase Chain Reaction
  • Recombination, Genetic


  • DNA, Mitochondrial