[Effects of hydrogen gas inhalation on serum high mobility group box 1 levels in severe septic mice]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 Sep;39(5):454-7.
[Article in Chinese]

Abstract

Objective: To investigate the effect of hydrogen gas inhalation on survival rate and serum high mobility group box 1 (HMGB1) levels in severe septic mice.

Methods: Severe sepsis was induced by cecal ligation and puncture (CLP) operation in mice.A total of 248 mice were randomly divided into four groups: sham operation group (sham), sham operation with hydrogen gas inhalation group (sham+H2), severe CLP group (severe CLP) and severe CLP with hydrogen gas inhalation group (severe CLP+H2). Hydrogen gas inhalation was given for 1 h at 1st and 6th h after CLP or sham operation, respectively. The survival rates and serum HMGB1 levels of all groups at different time points were measured.

Result: The 7-d survival rates of severe CLP mice was 0 % (Compared with Sham group, P <0.05), and the serum HMBG1 levels from h2 to h32 after CLP operation were significantly increased in severe CLP mice (Compared with Sham group, P <0.05). Hydrogen gas treatment increased the 7-d survival rate of severe CLP mice to 60 % (Compared with severe sepsis group, P <0.05) and significantly reduced the serum HMGB1 levels at different time points (Compared with severe sepsis group, P <0.05).

Conclusion: Hydrogen gas inhalation can decrease the serum HMGB1 levels and increase the survival rate of rats with severe sepsis.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Inhalation
  • Animals
  • Disease Models, Animal
  • HMGB1 Protein / blood*
  • Hydrogen / administration & dosage*
  • Hydrogen / therapeutic use
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Sepsis / blood*
  • Sepsis / drug therapy

Substances

  • HMGB1 Protein
  • Hydrogen